Grandma's Behavior While Pregnant
Affects Her Grandkids' Health
May 13, 2005; Page B1
Although life offers no guarantees, parents-to-be can increase their chances of having a healthy baby by, among other things, undergoing prenatal testing and making sure mom has a healthy pregnancy. But almost 2,500 years after Euripides noticed that "the gods visit the sins of the fathers upon the children," scientists are discovering that nature can be even crueler than the ancient Greek imagined: It can visit the sins of the grandparents on the children.
Such "transgenerational" effects are the latest focus of a growing field called fetal programming, or the fetal origins of adult diseases. It examines how conditions in the womb shape physiology in a way that makes people more vulnerable decades later to cardiovascular disease, diabetes, immune problems and other illnesses usually blamed on genetics or lifestyle, not on what arrived via the placenta. If a fetus is poorly nourished, for instance, it can develop a "thrifty phenotype" that makes it really good at getting the most out of every meal. After birth, that lets it thrive if food is scarce, but it's a recipe for Type 2 diabetes in a world of doughnuts and fries. Poor fetal nutrition can lead to hypertension, too: If it causes the fetus to produce too few kidney cells, the adult that the fetus will become won't be able to regulate blood pressure well.
Now, in a finding that seems to put our fate even further outside our control, researchers are seeing generation-skipping effects.
Last month, scientists reported that a child whose grandmother smoked while pregnant with the child's mother may have twice the risk of developing asthma as a child whose grandma didn't flood her fetus with carcinogens. Remarkably, the risk from grandma's smoking was as great as or greater than from mom's. Kids whose mothers smoked while pregnant were 1.5 times as likely to develop childhood asthma as children of nonsmoking moms. Kids whose grandmothers smoked while pregnant with mom were 2.1 times as likely to develop asthma, scientists reported in the journal Chest.
The harmful effects of tobacco, it seems, can reach down two generations even when the intervening generation -- mom -- has no reason to suspect her child may be at risk.
"Even if the mother didn't smoke, there was an effect on the grandchild," says Frank Gilliland of the University of Southern California, Los Angeles, who led the study of 908 children. "If smoking has this transgenerational effect, it's a lot worse than we realized."
What causes the grandma effect? One suspect is DNA in the fetus's eggs (all the eggs a girl will ever have are made before birth). Chemicals in smoke might change the on-off pattern of genes in eggs, including genes of the immune system, affecting children who develop from those eggs. Men whose mothers smoked don't seem to pass on such abnormalities, probably because sperm are made after birth.
Animal data hint at other grandma effects. Last week, scientists reported the first discovery that obesity and insulin resistance, as in Type 2 diabetes, can be visited on the grandkids of female rats that ate a protein-poor diet during pregnancy, lactation or both. Again, this occurred even when those rats' offspring, the mothers of the affected grandkids, were healthy, Elena Zambrano of the Institute of Medical Sciences and Nutrition, Mexico City, and colleagues report in the Journal of Physiology.
The findings, says Peter Nathanielsz of the University of Texas Health Sciences Center, San Antonio, "stretch the unwanted consequences of poor nutrition across generations."
In people, the type of "nutritional insult" to the fetus doesn't seem to matter. Too few calories, too little protein, too few other nutrients can all lead to diabetes, hypertension and other ills decades later. "That suggests that what links diet to adult diseases is something quite fundamental," says Simon Langley-Evans of the University of Nottingham, England. The key suspects: changes in DNA activity in the fetus or in the balance of hormones reaching it via the placenta.
Alarmingly, the list of what can be passed along to the next generation is growing. If you are undernourished as a first-trimester fetus, you won't pad your hips and thighs with enough fat tissue. If, as a child or adult, you take in more calories than you expend, the extras get stored in and around abdominal organs rather than on the thighs and hips, says Aryeh Stein of Emory University, Atlanta. One result is a body shaped like an apple (which brings a higher risk of heart disease). Another is a higher risk of gestational diabetes, in which blood glucose levels rise during pregnancy and too much glucose reaches the fetus. Babies born to moms with gestational diabetes have a higher risk of Type 2 diabetes.
When undernourished fetuses grow into adolescents, they don't respond as well to vaccines as babies who had a healthy gestation, scientists led by Thomas McCune of Northwestern University, Evanston, Ill., find. One reason may be that the third trimester is a critical time for development of the thymus, which produces the immune system's T cells. When immune-compromised girls become pregnant, they have less chance of having a healthy pregnancy and a healthy baby. Score another for the grandma effect.
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Dad's Hidden Influence
A father's legacy to a child's health may start before conception and last generations
Tina Hesman Saey
Pregnant women know the drill. Don't drink. Don't smoke. Don't eat too much fish. Take vitamins. Mothers have long shouldered the responsibility, and the blame, for their children's health. Fathers don't usually face the same scrutiny. How a man lives, where he works, or how old he is when his children are conceived doesn't affect
their long-term health, scientists used to think. But growing evidence suggests that a father's age and his exposure to chemicals can leave a medical legacy that lasts generations. Animal studies demonstrate that drugs, alcohol, radiation, pesticides, solvents, and other chemicals can lead to effects that are handed from father to son. Human studies are less clear, but some show that fathers play a role in fetal development and the health of their children.
Teenage dads face increased risk that their babies will be born prematurely, have low birth weight, or die at birth or shortly afterward, a new study in Human Reproduction shows. Babies of firefighters, painters, woodworkers, janitors, and men exposed to solvents and other chemicals in the workplace are more likely to be
miscarried, stillborn, or to develop cancer later in life, according to a review in the February Basic & Clinical Pharmacology & Toxicology. Fathers who smoke or are exposed at work to chemicals called polycyclic aromatic hydrocarbons put their children at risk of developing brain tumors. And, older fathers are more likely to have children with autism, schizophrenia, and Down syndrome and to have daughters who go on to develop breast cancer.
Though some of these observations are decades old, attitudes lag even further behind, says Cynthia Daniels, a political scientist at Rutgers University–New Brunswick in New Jersey. Dads aren't held accountable if something goes wrong during fetal development.
Matter of math
Since men make new sperm every 74 days, people used to reason, the genetic slate is wiped clean every couple of months. And even if a man makes defective sperm, the "all-or-nothing" view of reproduction holds that damaged sperm don't fertilize eggs. No harm. No foul. So no one bothers to remind men to protect themselves against environmental toxins. There are no images of "crack dads" and "crack babies" in the media like those of women who harm developing fetuses with drug and alcohol use, Daniels said in February at a meeting of the American Association for the Advancement of Science held in Boston. When someone does study fathers-to-be, the focus is usually on fertility, not on the consequences for children's health, she says.
Yet even fertility messages meet resistance from many men. Harry Fisch, director of the Male Reproductive Center and a urologist at Columbia University Medical Center, found that out when he suggested that men, like women, have ticking biological clocks. Men can produce sperm throughout life, but that doesn't mean their cells are forever young. "Every cell in the body ages," says Fisch. "Every cell. The older you get, the more chance of an abnormality. The same thing goes for sperm."
Men younger than 20 and older than 30 make more abnormal sperm than men in their 20s. These damaged sperm could create an unhealthy embryo or pass on damage that could lead to birth defects or illness in offspring.
It is not a popular message.
"Men do not want to hear this," Fisch says. "When my book came out, I got e-mails. I got faxes saying, 'How dare you say this? How can you say this? We know that there are men in their 70s having healthy children.'" Despite these anecdotal accounts of elderly dads, studies demonstrate that older men are at increased risk of passing on genetic abnormalities. It's a matter of math. Women are born with all the eggs they will produce in their lifetime. The cells that give rise to eggs divide 24 times, all before birth. But the cells that produce sperm continue to divide throughout a man's lifetime. Each year after puberty, a man's sperm-producing cells replicate about 23 times. Every time the cells divide is another chance for error. As a result, the sperm produced by a 40-year-old man have gone through about 610 rounds of replication. That's 610 chances of introducing a mutation in the DNA, or improperly divvying up genetic material.
Parents over age 40 are six times more likely to have children with Down syndrome than 25-year-old parents, Fisch and colleagues showed in a 2003 study in the Journal of Urology. An extra copy of chromosome 21 causes Down syndrome. This extra chromosome is just as likely to come from dad as mom in the older couples.
Older dads also have a higher risk of fathering children with rare mutations that cause dwarfism or a premature aging disease called Hutchinson-Gilford progeria syndrome. But sometimes aging fathers pass along traits that can't be traced to only a single mutation. Fathers 40 and older have an increased chance that their children will develop complex disorders such as autism or schizophrenia. There is growing evidence that those disorders are caused by defects in many genes and the way genes are turned off and on. Scientists don't yet understand the changes that age induces in sperm-making germ cells, and environmental exposure presents an even bigger mystery. People come in contact with a plethora of chemicals every day. But it is no easy task to sort out exactly which ones, or which combinations, cause heritable problems. The effects chemicals and radiation may have on offspring don't always follow predictable patterns either.
And when researchers do find a clear link between a father's lifestyle and his children's health, it's not always clear what the data mean. "What we can say is that we identified a group of fathers with adverse outcomes for their fetuses, but we don't have an idea of the mechanism," says Shi Wu Wen of the University of Ottawa in Canada and one of the lead authors of the study showing that babies of teenage fathers have a greater risk of birth problems. Wen and his colleagues examined birth records for more than 2.6 million babies born between 1995 and 2000 to married, first-time, 20-something mothers in the United States. Looking at the husbands' ages, the team found that babies of teenage fathers, but not middle-age men, had an elevated risk of still birth, low birth weight, and other birth problems.
The study was
online Feb. 6 in <http://dx.doi.org/10.1093/humrep/dem403>Human Reproduction.
Some animal studies showing paternal effects emerged years ago but were roundly dismissed, says Gladys Friedler, professor emeritus at Boston University.
OLDER AGE, HIGHER RISK. As men age, they stand a greater chance of fathering children who will develop schizophrenia by age 34. Paternal age is only one of many factors linked to schizophrenia. E. Roell, (Source: D. Malaspina, et al., Arch. of Gen. Psychiatry, 2001)
Four decades ago, Friedler was studying tolerance to narcotics, one of the first steps of addiction. To find out if a mother rat could pass tolerance on to her offspring along with antibodies and other immune factors, as some scientists theorized, Friedler exposed female rats to morphine before pregnancy. Babies of exposed mothers were born much smaller than average. And those babies also went on to give birth to tiny babies, even though the offspring had never encountered the drug. Friedler also gave male rats morphine before they bred. "To my total disbelief and bewilderment, paternal exposure also affected progeny," Friedler said at the AAAS meeting.
Her adviser dismissed the result. Morphine doesn't cause mutations, so the idea that males could hand down a trait without passing along a mutation seemed preposterous. The whole thing smacked of Lamarckism, the long-rejected idea that environmental influences can change an animal or plant's structure and offspring can inherit that change. But in recent decades, scientists have discovered that chemical modifications to DNA and proteins can change the way genes are packaged and regulated without changing the genes themselves. Such modifications are known as epigenetic changes. "What was Lamarckian is now epigenetic," Friedler says. Epigenetic modifications act as a molecular scrapbook, preserving memories of events in parents' lives and handing them down to the next generation and beyond.
"There's a chromosomal memory," says Anne Ferguson-Smith, a developmental geneticist at Cambridge University in England. "The chromosomes remember whether they came from the mother or the father." That memory is established in the form of a chemical mark called methylation. Methylation usually turns a gene off. At least 100 genes in humans are turned off only on the chromosome contributed by the mother or only on the chromosome that came from the father. Such genes
are called imprinted genes because of the indelible impression parents leave on their offspring's DNA. Several imprinted genes help build the placenta or encode growth factors that need to be tightly controlled so an embryo will develop correctly. "There's a contribution from both parents that is essential," Ferguson-Smith says. "One can't do without the other. They must work together to have a healthy offspring."
Imprints and other methylation marks are not encoded in the DNA. Instead the epigenetic modifications decorate chromosomes like ornaments on a Christmas tree. But these ornaments are heirlooms of a different type. It's as if a seedling grows straight from the ground already gussied up with tinsel and lights in the same places its parents were decorated. If a chemical or aging alters the epigenetic pattern on a man's chromosomes, his heirs could bequeath mismarked DNA to their children, too. Some mistakes may be as benign as exchanging a red bulb for a blue one. Other alterations, akin to placing the star on the lowest branch instead of the treetop, are likely to have more profound consequences. Male mice exposed to cocaine, for example, pass memory problems on to their pups, a 2006 study in Neurotoxicology and Teratology shows. The male mice inhaled cocaine in long daily sessions akin to crack binges. When they mated with females never given coke, they had pups that had trouble learning and remembering where to find food in simple mazes. The problem was especially severe for female offspring. The researchers couldn't find any obvious DNA damage in coke-smoking males' sperm, but did find altered levels of two enzymes involved in the methylation of DNA in sperm-producing tissue in the father mice. The result suggests that epigenetic changes may be responsible for the offspring's behavior problems.
Matthew Anway doesn't know whether the rats in his lab at the University of Idaho in Moscow have methylation problems. Some studies suggest they do, but Anway doesn't yet have definitive proof. He can prove that male rats exposed to a fungicide in the womb can pass tumors and diseases of the prostate and kidney down for at least three generations. The rats could provide the first model for how prostate disease is inherited, he says. Male babies born to mothers that had been injected with fungicide had prostate problems that mimic those seen during human aging. The second-generation rats also had more tumors, kidney defects, and higher rates of abscesses, cysts, and other infections than unexposed control rats. Germ cells in the testes of exposed rats also died more quickly than those in the control rats.
Subsequent generations of male rats also had the prostate and testes defects, and both male and female offspring developed kidney problems and tumors. But only male rats could pass along the defects. The exposed rats bequeathed their fungicide legacy to their sons, grandsons, and great-grandsons even though none of the later generations were exposed to the chemical.
Exposed animals decrease production of enzymes that methylate DNA, Anway says. But he hasn't yet found consistent changes in the methylation patterns in exposed rats. It's not clear whether Anway's results have any implication for human health. The rats were exposed to extremely high doses of fungicide through the completely unnatural route of injection. What's important is that the male shares experiences with descendants for years to come. Further research could give new insights, Anway says, into how alterations in early development could lead to adult disease in humans.
Anway, M.D., et al. 2005. Epigenetic transgenerational actions of endocrine disruptors and male fertility. Science 308(June 3):1466-1469.
Anway, M.D., S.S. Rekow, and M.K. Skinner. 2008.Transgenerational epigenetic programming of the embryonic testis transcriptome. Genomics 91(January):30-40.
Anway, M.D., and M.K. Skinner. 2008. Transgenerational effects of the endocrine disruptor vinclozolin on the prostate transcriptome and adult onset disease. The Prostate 68(April 1):517-529.
Chen, X.K., S.W. Wen, et al. In press. Paternal age and adverse birth outcomes: Teenager or 40+, who is at risk? Human Reproduction.Available at
Choi, J.-Y., et al. 2005. Association of paternal age at birth and the risk of breast cancer in offspring: A case control study. BMC Cancer 5(Oct. 31):143.
Cordier, S. 2008. Evidence for a role of paternal exposures in developmental toxicity. Basic & Clinical Pharmacology & Toxicology 102(February):176-181.
Cordier, S., et al. 2004. Parental exposure to polycyclic aromatic hydrocarbons and the risk of childhood brain tumors. American Journal of Epidemiology 159(June 15):1109-1116.
Croen, L.A., et al. 2007. Maternal and paternal age and risk of autism spectrum disorders. Archives of Pediatric and Adolescent Medicine 161(April):334-340.
El-Saadi, O., et al. 2004. Paternal and maternal age as risk factors for psychosis: Findings from Denmark, Sweden and Australia. Schizophrenia Research 67(April 1):227-236.
Fisch, H., et al. 2003. The influence of paternal age on Down syndrome. Journal of Urology 169(June):2275–2278.He, F., I.A. Lidow, and M.S. Lidow. 2006.
Consequences of paternal cocaine exposure in mice. Neurotoxicology and Teratology 28(Feb. 3):198–209.
Lewis, B.H., M. Legato, and H. Fisch. 2006. Medical implications of the male biological clock. Journal of the American Medical Association 296(Nov. 15):2369-2371.
Thacker, P.D. 2004. Biological clock ticks for men, too. Journal of the American Medical Association 291(April 14):1683-1685.
Skinner, M.K. 2008. What is an epigenetic transgenerational phenotype? F3 or F2. Reproductive Toxicology 25(January):2–6.
Frequently asked questions from the Epigenome Network of Excellence can be found at
University of Idaho
Department of Biological Sciences
Gibb Hall 239
P.O. Box 443051
Moscow, ID 83844-3051
University of Rennes I,
Campus de Beaulieu
Rennes cedex F-35042
Department of Political Science
Rutgers, the State University of New Jersey
89 George Street
New Brunswick, NJ 08901
Anne C. Ferguson-Smith
Department of Physiology, Development and Neuroscience
University of Cambridge
Physiology Building, G-floor Downing Street
Cambridge CB2 3EG
College of Physicians and Surgeons
Department of Urology
944 Park Avenue
New York, NY 10028
4 Newport Road #4
Cambridge, MA 02140
Shi Wu Wen
OMNI Research Group
Department of Obstetrics and Gynecology
University of Ottowa
501 Smyth Road, Box 241
Ottowa, Ontario K1H 8L6
>From Science News, Vol. 173, No. 13, March 29, 2008, p. 200.